A unicorn is a startup marked at USD 1 Billion or more. These are highly innovative and market disruptive startups. Czech Republic has more than 18.4K+ startups. Of these, 2 have become Unicorns, with the latest entrant to the club being Rohlik Group on Jul 01, 2021. In 2026, Czech Republic has seen no new unicorn startups.
Globally, Czech Republic is number 45 in terms of total Unicorns created, behind Chile (2 unicorns) at rank 44 and Bulgaria (2 unicorns) at rank 43. Karlin leads the list of cities in Czech Republic with most unicorns at 1. It is followed by Prague (1 unicorn). Among sectors, Consumer has seen the most unicorns created at 2, followed by Enterprise Applications at 1, and Enterprise Infrastructure at 1
Here is a list of all 2 unicorns created in Czech Republic:
2. Rohlik Group
Rohlik Group joined the Unicorn Club on Jul 01, 2021 after raising a $***** Series C funding round led by Index Ventures, valuing it at $*****.

Online grocery retailer. It allows users to purchase grocery products and daily essentials online through the platform. It offers fruits & vegetables, meat & seafood, dairy & bakery, drinks & beverages, and more. The company offers mobile applications for Android and iOS platforms.
Key facts about Rohlik Group
- Founded Year: 2014
- Location: Karlin (Czech Republic)
- Stage: Series D
- Primary Sector: Online Grocery
- Time to Unicorn: 7 years 6 months
- Top investors : European Investment Bank, Sofina
- Annual Revenue: €1B+ as on Apr 30, 2025
- Valuation: $*****
- Total Funding till date: $748M
- Employee Count: 345 as on Sep 30, 2026
- Latest Funding Round: Venture Debt, Jan 14, 2025, $*****
1. Avast

Developer of security and privacy software for computers and mobile. The company provides antivirus, VPN, cleanup, and driver update products. It offers solutions for home and business use, including protection for multiple devices. The company also provides online security and privacy tools, such as a secure browser and breach monitoring.
Key facts about Avast
- Founded Year: 1988
- Location: Prague (Czech Republic)
- Stage: Acquired
- Primary Sector: Cybersecurity and Mobile Utility Tools
- Time to Unicorn: 26 years 1 month
- Top investors : Herald Investment Management, Summit Partners
- Last Known Valuation: $*****
- Total Funding till date: $100M
- Employee Count: 819 as on Mar 31, 2026
- Latest Funding Round: Grant (prize money), Apr 01, 2023, $*****