About Waterline Ventures
Waterline Ventures is a venture capital firm founded in 2014. It is primarily based out of Cambridge, United States. As of May 2026, Waterline Ventures is an active investor, having invested in 36 companies, with 2 new investments in the last 12 months. It primarily invests in Series A round in United States based startups. Its investments are spread across a wide range of sectors from HealthTech to Enterprise Applications and FinTech. Most recently it participated in the $***** Seed round of CVRD Health Overall, Waterline Ventures portfolio has seen 1 IPO and 9 acquisitions including key companies like Amwell, Wellframe and HealthiPASS. A lot of funds co-invest with Waterline Ventures, with names like Asset Management Ventures sharing a substantial percentage of its portfolio. Waterline Ventures has team of 7 people including 1 partners.Key Metrics
Team Members
Portfolio Count
Portfolio Sectors
Portfolio Locations
Rounds of Entry
Series A, Seed & 1 more
Portfolio IPOs
Portfolio Soonicorns
Deals in last 12 months
Waterline Ventures' List of Top Investments
Waterline Ventures has a portfolio of 36 companies. Their most notable investments are in Nice Healthcare and evolvedMD.Their portfolio spans across United States, Canada and New Zealand. They have invested in HealthTech, Enterprise Applications, FinTech and 6 other sectors, across stages such as Series A, Seed and 1 more. Here is the list of top investments by Waterline Ventures:1. Manifold AI
Provider of an AI platform for life sciences research and development. The platform facilitates data analysis, collaboration, and governance for scientific teams. It offers tools for data engineering, cataloging, and visualization, along with pre-configured workspaces. The platform also provides access controls and infrastructure options to maintain data security and compliance. It supports various applications, including trusted research environments and data commercialization.
Key facts about Manifold AI
- Founded Year: 2016
- Location: Oakland (United States)
- Stage: Series B
- Total Funding till date: $40M
- Employee Count: 94 as on Aug 31, 2026
- Investors: Industry Ventures, TQ Ventures and 13 Others
- Latest Funding Round: Series B, Dec 18, 2025, $*****
- Highlight: Editors' Pick
2. Amwell
Provider of telehealth and consultation solutions for doctors and patients. It allows users to communicate with doctors for urgent care such as online therapy, nutrition counseling, psychiatry services, and many more. It also provides solutions for bronchitis, flu, sinus infection, urinary tract infections, and others. Doctors can schedule telehealth appointments and provide consultation for patients.
Key facts about Amwell
- Founded Year: 2006
- Location: Boston (United States)
- Annual Revenue: $100M - $500M as on Dec 31, 2025
- Stage: Public
- Total Funding till date: $887M
- Employee Count: 923 as on Apr 30, 2026
- Investors: HTIF, Philips and 25 Others
- Latest Funding Round: Series C, Aug 24, 2020, $*****
- Highlight: Public
3. Wellframe
Mobile and AI-based platform offering support to providers for better patient engagement. The Wellframe solution combines mobile devices and artificial intelligence with human care to provide patients with daily guidance and support while helping providers identify and patient needs and manage risk more dynamically across a population and over time. Additionally, care managers can use the information to provide more timely, up-to-date, and personalized feedback to their patients, in order to maximize patient adherence.
Key facts about Wellframe
- Founded Year: 2011
- Location: Boston (United States)
- Stage: Acquired
- Total Funding till date: $45.1M
- Employee Count: 38 as on May 31, 2026
- Investors: Threshold, F-Prime Capital and 14 Others
- Latest Funding Round: Series C, Nov 19, 2019, $*****
- Highlight: Acquired
Provider of an AI platform for clinical data management solutions. The platform offers tools for data capture, workflow management, and abstraction to deliver insights. It assists with registry data collection, improves workflow, and ensures data submission quality. The platform also supports research by streamlining screening and refining patient groups.
Key facts about Carta Healthcare
- Founded Year: 2017
- Location: San Mateo (United States)
- Valuation: $*****
- Stage: Series B
- Total Funding till date: $80.6M
- Employee Count: 133 as on Aug 31, 2026
- Investors: Storm Ventures, Maverick Ventures and 26 Others
- Latest Funding Round: Series B, May 08, 2025, $*****
- Highlight: Editors' Pick
5. Legacy
The company provides at-home sperm testing using its kits and analyses key fertility factors like semen volume, sperm count, concentration, motility, and morphology.
Key facts about Legacy
- Founded Year: 2014
- Location: Cambridge (United States)
- Valuation: $*****
- Stage: Series B
- Total Funding till date: $47.7M
- Employee Count: 433 as on Aug 31, 2026
- Investors: Valspring Capital, TQ Ventures and 19 Others
- Latest Funding Round: Series B, Jul 22, 2024, $*****
- Highlight: Editors' Pick
Waterline Ventures' Year-on-Year Investment Trends
Waterline Ventures has invested in 36 companies over the last 12 years, with an average of 2 new investments annually in the last 10 years. In 2025, it made 2 investments, while as of May 2026, it has made 2 investments in this year. Its most recent first time investment was in CVRD Health and most recent follow-on round was in evolvedMD.Year | No. of Investments | ||
|---|---|---|---|
1st Round | Follow-on | Total | |
2026 (YTD) | 2 | 0 | 2 |
2025 | 0 | 2 | 2 |
2024 | 2 | 0 | 2 |
2023 | 1 | 1 | 2 |
2022 | 3 | 2 | 5 |
2021 | 1 | 1 | 2 |
2020 | 3 | 1 | 4 |
2019 | 1 | 1 | 2 |
2018 | 1 | 2 | 3 |
2017 | 2 | 0 | 2 |
Waterline Ventures' Investments by Stage
Waterline Ventures has made 13 investments in Series A stage with an average round size of $13.9M, 9 investments in Seed stage with an average round size of $4.66M and 1 investment in Series B stage with an average round size of $16M.Stage of entry | No. of Investments |
|---|---|
Series A | 13 |
Seed | 9 |
Series B | 1 |
Note: We have considered here, only first round of investments
Waterline Ventures' Investments by Sector
Waterline Ventures has a diverse portfolio, with companies operating in the HealthTech, Enterprise Applications, Vertical SaaS, FinTech and Healthcare. Notably, it has invested in 32 Tech companies, 28 Enterprise (B2B) companies, 25 Software companies and at least 13 companies focusing on Saas.Sector | No. of Investments |
|---|---|
HealthTech | 18 |
Enterprise Applications | 15 |
Vertical SaaS | 4 |
FinTech | 3 |
Healthcare | 3 |
Others | 9 |
Note: We have considered here, only first round of investments
Waterline Ventures' Investments by Geography
Waterline Ventures has made most investments in United States (22), followed by Canada where it has made 1 investment.Country | No. of Investments |
|---|---|
United States | 22 |
Canada | 1 |
Note: We have considered here, only first round of investments
Waterline Ventures' recent investments
Waterline Ventures has made 2 investments in 2026 so far - CVRD Health and Kinomatic.Here are the most recent investments by Waterline Ventures:
Filter this listDate | Company | Location | Round Details | Round Amount | Co-Investors |
|---|---|---|---|---|---|
May 21, 2026 | United States | Seed | 5808 | [+1] | |
Apr 01, 2026 | United States | Seed | 9141 | - | |
Jul 14, 2025 | United States | Series B | 4039 | [+4] | |
Feb 04, 2025 | United States | Seed | 9953 | [+5] | |
Aug 21, 2024 | United States | Series A | 9493 | [+2] |
IPOs and Publicly Listed companies in Waterline Ventures' Portfolio
1 of Waterline Ventures' portfolio company became public - Amwell. It got listed on the National Association of Securities Dealers Automated Quotations (NASDAQ), in Sep 2020 at marketcap of $742MHere are Waterline Ventures' portfolio companies that went public:
Company | IPO Date | First Invested on | First Round of Investment | Round Size |
|---|---|---|---|---|
Sep 17, 2020 | - | - | 1022 |
Acquired companies in Waterline Ventures' Portfolio
9 companies from Waterline Ventures' portfolio have been acquired. The most recent acquisition was Cured in Jan 2024 by Innovaccer for $*****.Here are Waterline Ventures' portfolio companies that got acquired most recently:
Company | Acquisition Date | First Invested on | First Round of Investment | Round Size |
|---|---|---|---|---|
Jan 16, 2024 | Oct 03, 2019 | Seed | 4369 | |
May 24, 2023 | Jan 04, 2016 | Series A | 4560 | |
Oct 21, 2021 | Dec 22, 2014 | Seed | 5828 | |
Oct 19, 2021 | Sep 08, 2014 | Series A | 1302 | |
Dec 17, 2020 | Dec 06, 2017 | Series A | 5147 |
Team profile of Waterline Ventures
Waterline Ventures has a team of 7 members including 1 Partner and 1 Principal located in United States. Waterline Ventures' team does not sit on the board of any company as of now.Co-investors of Waterline Ventures
Over the past 12 years, 161 investors have co-invested in Waterline Ventures's portfolio companies. This includes funds and angels.
- Invested before Waterline Ventures: Rock Health, Draper and 29 others have invested in rounds before Waterline Ventures. There are 2 companies where Rock Health has invested before Waterline Ventures and 1 company where Draper has invested before Waterline Ventures.
- Top Co-investors of Waterline Ventures: 52 investors entered a company along with Waterline Ventures. These include investors like Asset Management Ventures (2 companies).
- Invested after Waterline Ventures: A total of 78 investors have invested in Waterline Ventures's portfolio after their investments. Top Investors include FCA Venture Partners (2 companies), DNA Capital (2 companies) and F-Prime Capital (1 company).
Recent News related to Waterline Ventures
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evolvedMD Secures $34M to Expand Integrated Behavioral Health Care NationwideHIT Consultant•Jul 15, 2025•evolvedMD, Conductive Ventures, FCA Venture Partners, Healthworx and 3 others
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SimpliFed Raises $4M in FundingFinSMEs•Feb 06, 2025•SimpliFed, Morningside, The Artemis Fund, Cultivation Capital and 4 others
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Scottsdale-based EvolvedMD raises $14 million to expand workforce, footprintThe Business Journals•Jun 15, 2023•evolvedMD, Conductive Ventures, FCA Venture Partners, Tectonic Ventures and 1 other
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